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454 life sciences platform  (Roche)


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    Structured Review

    Roche 454 life sciences platform
    454 Life Sciences Platform, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/454+life+sciences+platform/us12049667-1643-7-11
    Average 86 stars, based on 1 article reviews
    454 life sciences platform - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Amplification:

    Article Title: Evolution of HIV-1 tropism at quasispecies level after 5 years of combination antiretroviral therapy in patients always suppressed or experiencing episodes of virological failure.
    Article Snippet: Conventional sequencing was performed with the ABI Prism 310 instrument, using the BigDye Terminator cycle sequencing kit (Applied Biosystems). .. UDPS was performed with the 454 Life Sciences platform (GS-FLX, Roche Applied Science, Monza, Italy), using Titanium chemistry.15,17 V3 amplification was performed by nested PCR. .. Briefly, two rounds of 30 cycles (948C for 2 min, 948C for 30 s, annealing at 608C for 30 s, extension at 688C for 30 s and final elongation at 688C for 5 min) were carried out using a proof-reading DNA polymerase (Platinumw Taq DNA Polymerase High Fidelity, Invitrogen, by Life Technologies, Monza, Italy).

    Nested PCR:

    Article Title: Evolution of HIV-1 tropism at quasispecies level after 5 years of combination antiretroviral therapy in patients always suppressed or experiencing episodes of virological failure.
    Article Snippet: Conventional sequencing was performed with the ABI Prism 310 instrument, using the BigDye Terminator cycle sequencing kit (Applied Biosystems). .. UDPS was performed with the 454 Life Sciences platform (GS-FLX, Roche Applied Science, Monza, Italy), using Titanium chemistry.15,17 V3 amplification was performed by nested PCR. .. Briefly, two rounds of 30 cycles (948C for 2 min, 948C for 30 s, annealing at 608C for 30 s, extension at 688C for 30 s and final elongation at 688C for 5 min) were carried out using a proof-reading DNA polymerase (Platinumw Taq DNA Polymerase High Fidelity, Invitrogen, by Life Technologies, Monza, Italy).

    Next-Generation Sequencing:

    Article Title: High-throughput polynucleotide library sequencing and transcriptome analysis
    Article Snippet: .. Suitable next generation sequencing technologies include the 454 Life Sciences platform (Roche, Branford, CT) (Margulies et al., Nature, 437, 376-380 (2005)); Illumina's Genome Analyzer, GoldenGate Methylation Assay, or Infinium Methylation Assays, i.e., Infinium HumanMethylation 27K BeadArray or VeraCode GoldenGate methylation array (Illumina, San Diego, CA; Bibkova et al, Genome Res. ..

    Methylation:

    Article Title: High-throughput polynucleotide library sequencing and transcriptome analysis
    Article Snippet: .. Suitable next generation sequencing technologies include the 454 Life Sciences platform (Roche, Branford, CT) (Margulies et al., Nature, 437, 376-380 (2005)); Illumina's Genome Analyzer, GoldenGate Methylation Assay, or Infinium Methylation Assays, i.e., Infinium HumanMethylation 27K BeadArray or VeraCode GoldenGate methylation array (Illumina, San Diego, CA; Bibkova et al, Genome Res. ..

    Polymerase Chain Reaction:

    Article Title: Challenges with using primer IDs to improve accuracy of next generation sequencing.
    Article Snippet: In addition, the Agilent 2100 Bioanalyzer (Agilent Life Science, Santa Clara, California, US) was used to verify the quality and length of the amplicons. .. After quality controls, PCR amplicons from the different samples were pooled and sequenced in both forward and reverse direction on the 454 Life Sciences platform (GS-FLX Titanium, Roche Applied Science) according to the manufacturer’s instructions. .. Sanger sequencing The PCR products from all samples were also subjected to population Sanger sequencing (ABI Prism 3100) using the Big Dye terminator cycle sequencing kit according to recommendations by the manufacturer (Life technologies, Foster City, California, US).

    Sequencing:

    Article Title: Resistant mutations and quasispecies complexity of hepatitis B virus during telbivudine treatment.
    Article Snippet: .. UDPS in this study was performed on the 454 Life Sciences platform (GS-FLX; Roche Applied Science) in both directions of the sequence according to the manufacturer’s instructions. ..

    Article Title: 'Sentinel' mutations in standard population sequencing can predict the presence of HIV-1 reverse transcriptase major mutations detectable only by ultra-deep pyrosequencing.
    Article Snippet: .. UDPS was carried out using the 454 Life Sciences platform (GS-FLX; Roche Applied Science), using the HIV prototype primer set and sequencing protocol of the 2009 HIV Alphasite Study, supported by Roche.21 Briefly, three overlapping cDNA sequences provided the source material for eight partially overlapping amplicons spanning the entire protease and RT codons, which were generated using barcoded primers. .. PCR products were then clonally amplified on capture beads in water-in-oil emulsion micro-reactors, and pyrosequencing was performed, following the standard approach for PCR amplicon sequencing.

    Generated:

    Article Title: 'Sentinel' mutations in standard population sequencing can predict the presence of HIV-1 reverse transcriptase major mutations detectable only by ultra-deep pyrosequencing.
    Article Snippet: .. UDPS was carried out using the 454 Life Sciences platform (GS-FLX; Roche Applied Science), using the HIV prototype primer set and sequencing protocol of the 2009 HIV Alphasite Study, supported by Roche.21 Briefly, three overlapping cDNA sequences provided the source material for eight partially overlapping amplicons spanning the entire protease and RT codons, which were generated using barcoded primers. .. PCR products were then clonally amplified on capture beads in water-in-oil emulsion micro-reactors, and pyrosequencing was performed, following the standard approach for PCR amplicon sequencing.



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